(2005) also demonstrated that only these three sperm parameters are correlated with the non-return rate when studying the relationship between sperm kinetics and in vivo fertility. Boar semen containing spermatozoa exhibiting increased VSL and LIN is associated with larger litter sizes after insemination (Holt et al., 1997 Estienne et al., 2007).įurthermore, farrowing rate is related to VCL, LIN and hyperactivated sperm (Oh et al., 2010). Several studies have previously reported significant beneficial effects of the linearity (LIN) indicator in swine reproduction field trials. It appeared that the concentration of Minitube Equex paste at 1.9% gave a better result than Group II (1.5%), but was not significantly different at 1.7% or from the control (1.5% Nova Equex STM paste). Likewise, the WOB kinetic indicator of Group IV was the highest among all groups.Īfter thawing at 1 h, the linearity (LIN) in Group IV showed a higher value than in Group II, but this did not differ significantly from the other groups.
Nevertheless, the total motility and progressive motility in Group IV (1.9% Minitube Equex paste) was higher than in Group II (1.5%) and Group III (1.7%) after thawing at 0 h and higher than in Group I (1.5% Nova Equex STM paste control).
Consistently in this study, the motility characteristics of frozen-thawed spermatozoa decreased gradually over the incubation times of 0, 1 and 2 h. This would seem to be an important contribution to their relatively poor fertilising potential when introduced into the reproductive tract during artificial insemination (Watson, 2000). The majority of samples showed a variable degree of impairment, while the minority exhibited vigorous forward progressive motility. P 0.05).Ĭryopreserved spermatozoa are characterised by a decline in motility. Least-square means were obtained from the statistical models and compared using the least significant difference test. The statistical models included a fixed effect of group (I, II, III, IV), time at 0 (10 min post-thawing), 1 and 2 h, and the two-way interaction.īoar was included in the models as a random effect. All sperm motility characteristics were analysed by multiple ANOVA using the general linear mixed model procedure (MIXED) of SAS. Statistical analyses were performed using SAS (SAS version 9.0, Cary, NC, USA). In Group IV after thawing at 0 h, rapid velocity and the velocity curved line were significantly higher than in Groups II and III (P 45 progressive (STR>), 45 connectivity (pixels), 11 velocity average path (VAP) points (pixels), 5.Īverage speed indices of sperm quality assessment, i.e., total motility, progressive motility, rapid velocity, velocity curved line (VCL, μm/s), velocity straight line (VSL, μm/s), VAP (μm/s), linearity (LIN, as a measure of a curvilinear path, VSL/VCL, %), straightness (STR, as the linearity of the average path, VSL/VAP, %), wobble (WOB, oscillation measure of the actual path from the average path, VAP/VCL, %), amplitude of lateral sperm head (ALH, μm) and beat cross frequency (BCF, Hz), were determined using CASA. Groups II, III and IV were the experiment groups, and contained different concentrations of Minitube Equex paste (Group II: 1.5% Group III: 1.7% and Group IV: 1.9%) added to the freezing extender III.Īfter freezing and thawing, sperm motility characteristics were evaluated by Sperm Class Analyzer ® incubated at 37 ☌ for 0 (10 min), 1 and 2 h post-thawing. Group I was the control and included only 1.5% Nova Equex STM paste.
Minitube microscope parts plus#
Each ejaculate was aliquoted and cryopreserved in base freezing extender III as Tris-citrate egg yolk (TEY) extender plus 9.0% glycerol classified into four groups. The aim of the present study was to determine the optimal concentration of Minitube Equex paste (Minitube, Tiefenbach, Germany) for boar semen cryopreservation in comparison with Nova Equex STM paste (control).įifteen ejaculates from 12 mature boars were collected by the glove-hand method. However, Equex paste produced by Nova Chemical Sales Inc. Abstract Introduction Materials and Methods Results Discussion Acknowledgments References SažetakĮquex paste is a non-permeating cryoprotective agent (CPA) that improved post-thaw survival of spermatozoa during boar semen cryopreservation.